Upon completion of the procedure, each animal was rewarmed to 35°C and weaned from the veno-RV ECMO, the lungs were allowed to inflate with mechanical ventilation, and the veno-RV ECMO system was disconnected after 30 min of bypass weaning. After removing the veno-RV ECMO cannulas, the right jugular and right femoral veins were repaired with 6-0 Prolene sutures (Ethicon Inc). All blood that remained in the venous tubing and the oxygenator after veno-RV ECMO was collected and reinfused.
Next, the chest was irrigated with normal saline solution, a 28F chest drainage tube was inserted into the thorax through the trocar hole, and a suction device was connected. The wound was closed in layers and the skin was sutured in a subcuticular fashion. Each animal was allowed to breathe spontaneously; once it could breathe room air with adequate oxygenation, the chest drainage tube was removed, and the animal was weaned from ventilation and extubated.
Hemodynamic measurements and blood samples were obtained under control conditions, during veno-RV ECMO, and up to the end of the experiment. The initial base excess (BE) was corrected to within the normal range by IV sodium bicarbonate administration and further corrections were made, as necessary, up to the end of the experiment. The animals were given 20 mg/kg of IM cefazolin sodium postoperatively and then maintained for 14 days on a daily dose of 40 mg/kg of amoxicillin, an oral antibiotic drug. Sixty days after surgery, each dog underwent fiber bronchoscopic examination under anesthesia induced with IM ketamine. www.mycanadianpharmacy.com
Each value is expressed as the mean±SD. The data were compared with the paired t test, and differences were considered significant at p<0.05.
The experimental protocol was approved by the Animal Care and Use Committee of Saga Medical School. The animals received humane care in compliance with the “Principles of Laboratory Animal Care,” formulated by the National Society for Medical Research, and the “Guide for the Care and Use of Laboratory Animals,” published by the National Institutes of Health (NIH publication No. 86-23, revised 1985).